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Contrasted survival under field or controlled conditions displays associations between mRNA levels of candidate genes and response to OsHV-1 infection in the Pacific oyster Crassostrea gigas ArchiMer
Normand, Julien; Li, Ronghua; Quillien, Virgile; Nicolas, Jean-louis; Boudry, Pierre; Pernet, Fabrice; Huvet, Arnaud.
Pacific oyster Crassostrea gigas suffers from chronic or sporadic mortality outbreaks worldwide, resulting from infectious diseases and/or physiological disorders triggered by environmental factors. Since 2008, ostreid herpesvirus OsHV-1 μVar has been identified as the main agent responsible for mass mortality of juvenile oysters in Europe. Previous studies of genome-wide expression profiling have provided candidate genes that potentially contribute to genetically-based resistance to summer mortality. To assess their value in determining resistance to the juvenile mass mortality that has occurred in France since 2008, we analyzed the expression of 17 candidate genes in an experimental infection by OsHV-1 μVar, and in an in vivo field experiment. Individual...
Tipo: Text Palavras-chave: Molluscs; Gene expression profiling; Inhibitor of kappa B; Summer mortality; Ostreid herpesvirus.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00177/28823/27325.pdf
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Evidence of herpesvirus (OsHV-1) resistance in juvenile Crassostrea gigas selected for high resistance to the summer mortality phenomenon ArchiMer
Degremont, Lionel.
During the MOREST project (2001–2006), oyster summer mortality was extensively investigated in Crassostrea gigas in France. However, significant increases in magnitude and geographic distribution of summer mortality episodes have been reported since 2008. This paper investigates the response to selection for survival, which was successfully performed between 2001 and 2003, in this new context. It also examines the relationship between the mortality and the three pathogens Vibrio aestuarianus, V. splendidus and the Ostreid herpesvirus 1 (OsHV-1). Three batches of juvenile (6-month-old) Crassostrea gigas were produced in February 2009 and tested in the field in the Marennes-Oléron Bay during summer (August and September) 2009: one control batch, one batch...
Tipo: Text Palavras-chave: Summer mortality; Crassostrea gigas; Disease resistance; Ostreid herpesvirus; Vibrio sp; Oyster.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00033/14472/11770.pdf
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Size and genotype affect resistance to mortality caused by OsHV-1 in Crassostrea gigas ArchiMer
Degremont, Lionel.
As with summer mortalities reported in France between 2001 and 2006, mortality caused by the Ostreid herpesvirus 1 (OsHV-1) in Crassostrea gigas affects mostly juveniles, although adults can also be impacted to a small extent. This could suggest that both mortalities have similar causes and that establishment of resistance, in particular to the Ostreid herpesvirus 1 (OsHV-1), depends on either the size or the age of oysters. The present study reports an investigation of both size and age using three cohorts produced during winter and three produced during summer. Each cohort contained oysters genetically selected to be resistant or susceptible to the summer mortality phenomenon, as well as unselected control oysters. Any abnormal mortality was recorded...
Tipo: Text Palavras-chave: Mortality; Crassostrea gigas; Disease resistance; Size; Age; Ostreid herpesvirus.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26799/24909.pdf
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